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well tissue culture plates (BD Biosciences). MCF A-EV cells, MCF A K-RasV12 cells, or MCF10A-K-RasV12 cells (7.0 105)pretreatedwithsiRNA(72h)orMEKinhibitors(24 h) were detached and seeded in the upper chamber of the inserts for these assays. Cells that invaded through the mem-brane overnight were counted in four separate fields in three

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